Expression,purification and bioimformatics analysis of allergen protein Profilin from silkworm (Bombyx mori) / 中国免疫学杂志
Chinese Journal of Immunology
; (12): 81-84, 2017.
Article
in Zh
| WPRIM
| ID: wpr-508448
Responsible library:
WPRO
ABSTRACT
Objective:
To obtain recombinant Profilin of silkworm,identify its immunogenicity,predict its B cell epitopes and construct the evolutionary trees.Methods:
The nucleotide sequence of Profilin was acquired from NCBI,synthesized it and cloned it into pET-28 vector. Then,the recombinant plasimids were transformed to E. coli BL21. After induced by IPTG,recombinant protein was purified by Affinity chromatography. Furtherly,its allergenicity was identified by Western blot,the potential B cell epitopes was analyzed through DNAStar and build the evolutionary trees by MEGA5. 05.Results:
The recombinant protein of Profilin was successfully expressed and purified by affinity chromatography. Besides,the protein contains a high IgE-binding activity with IgE existing in serum of patients allergic to silkworm.Conclusion:
The recombinant proflilin has IgE-binding activity, and it is meaningful for fundamental research and specific diagnosis studies of allergic diseases caused by silkworm.
Full text:
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Index:
WPRIM
Language:
Zh
Journal:
Chinese Journal of Immunology
Year:
2017
Type:
Article