Simultaneous determination of seven constituents in Sangqishouwu Tablets by HPLC / 中成药
Chinese Traditional Patent Medicine
; (12): 737-740, 2017.
Article
in Zh
| WPRIM
| ID: wpr-512725
Responsible library:
WPRO
ABSTRACT
AIM To establish an HPLC method for the simultaneous content determination of calycosin-7-glucoside,ononin,calycosin,formononetin,oxypaeoniflorin,alibiflorin and benzoylpaeoniflorin in Sangqishouwu Tablets (Talxilli Herba,Astragali Radix,Polygoni multiflori Radix Praeparata,etc.).METHODS The analysis of methanol extract of this drug was performed on a 25 ℃ thermostatic Diamonsil C18 colmn (250 mm × 4.6 mm,5 μm),with the mobile phase comprising of acetonitrile-0.02% phosphoric acid flowing at 0.9 mL/min in a gradient elution manner,and the detection wavelengths were set at 230 nm and 254 nm.RESULTS Seven constituents showed good linear relationships within their own ranges (r ≥ 0.999 2),whose average recoveries were 97.13%-100.03% with the RSDs of 0.69%-1.47%.CONCLUSION This sensitive,accurate and specific method can be used for the rapid quality control of Sangqishouwu Tablets.
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WPRIM
Language:
Zh
Journal:
Chinese Traditional Patent Medicine
Year:
2017
Type:
Article