Carnosic acid inhibits TLR4-MyD88 signaling pathway in LPS-stimulated 3T3-L1 adipocytes
Nutrition Research and Practice
; : 516-520, 2014.
Article
in En
| WPRIM
| ID: wpr-51351
Responsible library:
WPRO
ABSTRACT
BACKGROUND/OBJECTIVES:
Carnosic acid (CA), found in rosemary (Rosemarinus officinalis) leaves, is known to exhibit anti-obesity and anti-inflammatory activities. However, whether its anti-inflammatory potency can contribute to the amelioration of obesity has not been elucidated. The aim of the current study was to investigate the effect of CA on Toll-like receptor 4 (TLR4) pathways in the presence of lipopolysaccharide (LPS) in 3T3-L1 adipocytes. MATERIALS/METHODS:
3T3-L1 adipocytes were treated with CA (0-20 microM) for 1 h, followed by treatment with LPS for 30 min; mRNA expression of adipokines and protein expression of TLR4-related molecules were then measured.RESULTS:
LPS-stimulated 3T3-L1 adipocytes showed elevated mRNA expression of tumor necrosis factor (TNF)-alpha, interleukin-6, and monocyte chemoattractant protein-1, and CA significantly inhibited the expression of these adipokine genes. LPS-induced up regulation of TLR4, myeloid differentiation factor 88, TNF receptor-associated factor 6, and nuclear factor-kappaB, as well as phosphorylated extracellular receptor-activated kinase were also suppressed by pre-treatment of 3T3-L1 adipocytes with CA.CONCLUSIONS:
Results of this study suggest that CA directly inhibits TLR4-MyD88-dependent signaling pathways and decreases the inflammatory response in adipocytes.Key words
Full text:
1
Index:
WPRIM
Main subject:
Phosphotransferases
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RNA, Messenger
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Up-Regulation
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Interleukin-6
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Tumor Necrosis Factor-alpha
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Adipocytes
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Chemokine CCL2
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TNF Receptor-Associated Factor 6
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Toll-Like Receptor 4
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Myeloid Differentiation Factor 88
Language:
En
Journal:
Nutrition Research and Practice
Year:
2014
Type:
Article