Three-dimensional culture and differentiation of biological bone tissue and itsin vivo repair effect in an animal model / 中国组织工程研究

ABSTRACT

BACKGROUND:In vitro culture of tissue-engineered bone is an important method for bone repair. Three-dimensional (3D) printed bone stents combined with bioreactor culture are of significance in bone tissue engineering. OBJECTIVE:To study thein vivo repair effect of the 3D printed biomaterial scaffold with human mesenchymal stem cells (hMSCs) cultured in bioreactor. METHODS:The scaffold was constructed by poly(lactic-co-glycolic acid)/hydroxyapatite (PLGA/HA)via 3D printing and freeze-dying techniques, and then hMSCs were seeded onto the scaffold and cultured in bioreactors. Al rabbits were numbered and divided into control (No.1 and 2), experimental 1 (No. 3 and 4) and experimental 2 (No. 5 and 6) groups, and each group had two subgroups positive and negative. The rabbit left distal femur in each group was modeled into bone defect and the single PLGA/HA scaffold, PLGA/HA scaffold carrying non-induced hMSCs were implanted in the positive and negative groups of the control group, respectively; the PLGA/HA-201405-1 and PLGA/HA-201405-2carrying induced hMSCs were implanted into the positive and negative subgroups of the experimental 1 and 2 groups, respectively. Additionaly, the right femur in the experimental 2 group was driled only. The osteogenesis ability and biodegradability were determined using electron microscope, thein vivorepair was observed through CT examination, and the histopathological examination was performed after bone healing. RESULTS AND CONCLUSION:The scaffold with topological structure suitable for cellseeding was prepared. A large number of new calcium nodules were observed under electron microscope in the experimental groups indicating overt achievement in bone healing. These results suggest that the prepared scaffold achieves a good repair effect preliminarily.