Role of endotoxemia in the development of hepatocarcinoma in rats / 中国病理生理杂志

ABSTRACT

AIM: To explore the effect of endotoxemia on hepatocarcinoma course induced by TAA. METHODS: The rat cirrhosis was induced by intaking TAA (0.03%) for four months, hepatocarcinom for six months. Lipoplysaccharide (100 ?g/kg) was administered subcutaneously every two days from the fifth month to the end of sixth month in TAA-treated rats. The plasma endotoxin content, ?-glutamyl transpeptidase(?-GT) activity, DNA index and aneuploidy index were determined, the point mutation of N-ras, p53 gene was detected in hepatocarcinoma rats. RESULTS: Endotoxin increased protein overexpression of p53, bcl-2 and N-ras , p53 gene mutation point and free radical production, reduced antioxidase activity and aggravated DNA damage in hepatocarcinoma rats. CONCLUSION: Endotoxin could accelerate hepatocarcinogenesis promoted by TAA.