mRNA Expression of Caspase-1 and Caspase-3 in the Peripheral Blood Mononuclear Cells of Patients with Systemic Lupus Erythematosus / 中华皮肤科杂志

ABSTRACT

Objective To investigate the mRNA espression level of Caspase-1 and Caspase-3 in the patients with systemic lupus erythematosus(SLE) and its association with lymphocyte apoptosis. Methods Reverse transcription-polymerase reaction (RT-PCR) technique was applied to semiquantitatively analyze the mRNA expression of Caspase-1 and Caspase-3 in the peripheral blood mononuclear cells (PBMC) from 34 active SLE patients and 30 healthy subjects. Results Compared with normal controls, the mean levels of Caspase-1 or Caspase-3 mRNA expression in active SLE group was significantly increased(P0.05). Conclusion There are increased expressin of Caspase-1 and Caspase-3 in the PBMC of SLE patients, which may be involved in the lymphocyte apoptosis of SLE patients.