Serum immunopharmacological assessment:effects of cycloheximide on the activation of T lymphocytes of mouse / 中国病理生理杂志
Chinese Journal of Pathophysiology
; (12)1989.
Article
in Zh
| WPRIM
| ID: wpr-519113
Responsible library:
WPRO
ABSTRACT
AIM: To investigate the effect of cycloheximide on the T cells activation by mitogen in vitro with CD69 expression as activation marker for the application of this drug clinically. METHODS:Lymphocytes were isolated from lymphoid nodes of C57BL/6 mouse. The cells were preincubated with cycloheximide(CHX), 5% serum containing CHX respectively for an hour, then further incubated with polyclonal activators (Con A or PDB). Harvesting the cells after whole incubation for 24 h, we estimated the expression rates of CD69 on T cells by flow cytometry following two-color immunofluorescent staining. RESULTS: The expression rates of CD69 on the T cells preincubated with CHX, serum containing CHX after the stimulation in response to Con A or PDB all showed significant difference with the expression rates of control group, respectively ( P
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Index:
WPRIM
Language:
Zh
Journal:
Chinese Journal of Pathophysiology
Year:
1989
Type:
Article