Determination of Enalapril in Human Plasma With HPLC-MS / 中国药房
China Pharmacy
; (12)2001.
Article
in Zh
| WPRIM
| ID: wpr-520054
Responsible library:
WPRO
ABSTRACT
OBJECTIVE:
To estabilish a HPLC-MS method for determining enalapril in human plasmaMETHODS:
Alprozalam was added into plasma sample as an internal standard,then supernate of the sample was extracted through solid-phase extration column,washed with methanol and detected by HPLC-MSmethod:
column,ODS C18;mobile phase,methanol-0 01% formic acid(45∶55);flow rate,0 8ml/min;capillary voltage,3 81kV;cone voltage,39 0V The selected ion was determined by ESTRESULTS:
The calibration curve was linear within the range of 2 5~400ng/ml r=0 9 996,the recovery was 102 2%,RSDs of intra -day and inter-day were 4 0% and 5 4%,respectivelyCONCLUSION:
The method is accurate and sensitive with no endogenous interference It can be applied to studying the pharmacokinetics and bioavailability of enalapril tablets in humans
Full text:
1
Index:
WPRIM
Language:
Zh
Journal:
China Pharmacy
Year:
2001
Type:
Article