Selective inhibition of glutamate uptake by mercury in cultured mouse astrocytes
Yonsei Medical Journal
;
: 299-305, 1995.
Article
in English
| WPRIM
| ID: wpr-52250
ABSTRACT
We studied the effects of organic and inorganic mercury (Hg) on the uptake of L-[3H] glutamate (L-GLU) in cultured mouse astrocytes. Following exposure to mercuric chloride (MC) [0.2 approximately 5.0 microM], selective and dose-dependent inhibition of L-GLU uptake to 50% of control levels was observed, whereas 2-deoxyglucose (2-DG) uptake was not significantly affected. Methylmercuric chloride (MMC) also inhibited L-GLU uptake but 50% reduction was reached only at a concentration of 10 microM. Inhibition of L-GLU uptake by MMC appears to be closely linked to voltage-sensitive calcium channels as evidenced by the lack of L-GLU uptake inhibition by MMC in calcium-free medium or in the presence of the channel blocker verapamil. Exposure to a variety of divalent metallic ions, including CuCl2, FeCl2 and ZnCl2, did not affect L-GLU uptake in astrocytes in vitro. Exposure to PbCl2, however, resulted in a decline in L-GLU uptake, though to a much smaller degree than that observed with Hg compounds. Selective impairment of astroglial L-GLU transport may represent a critical early pathogenetic feature of Hg-induced neurotoxicity.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Cells, Cultured
/
Astrocytes
/
Glutamic Acid
/
Animals
/
Mercury
/
Mice, Inbred C57BL
Limits:
Animals
Language:
English
Journal:
Yonsei Medical Journal
Year:
1995
Type:
Article
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