Role of TGF-?_1 and its signaling transduction molecule Smad4 in the development of glomerulosclerosis / 中国病理生理杂志
Chinese Journal of Pathophysiology
; (12)2000.
Article
in Zh
| WPRIM
| ID: wpr-525372
Responsible library:
WPRO
ABSTRACT
AIM:
To explore the role of TGF-?_1 and signaling transduction molecule, Smad4, in the development of glomerulosclerosis.METHODS:
Expression levels of TGF-?_1, Smad4, collagen Ⅰ proteins were evaluated by immunohistochemistry in renal biopsies from 38 cases with a spectrum of glomerulonephritis, and compared with 20 normal kidney tissue with image analysis system. After stimulation with TGF-?_1, expressions of endogenous Smad4 and collagen Ⅰ mRNA and proteins and its modulation by TGF?_1 were evaluated by RT-PCR and Western blotting analyses in cultured human mesangial cells.RESULTS:
All types of proliferative and sclerotized glomerulonephritis showed an increased expression of TGF-?_1, Smad4 and collagen Ⅰ ompared with the 20 normal kidney tissue in glomerular (P
Full text:
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Index:
WPRIM
Language:
Zh
Journal:
Chinese Journal of Pathophysiology
Year:
2000
Type:
Article