Effect of curcumin on heme oxygenase -1 expression in the lung of rats treated with LPS / 中国病理生理杂志
Chinese Journal of Pathophysiology
; (12)2000.
Article
in Zh
| WPRIM
| ID: wpr-525943
Responsible library:
WPRO
ABSTRACT
AIM:
To study the regulatory effect of curcumin on expression of heme oxygenase-1 (HO-1) in the lung of rat treated with LPS.METHODS:
Eighteen rats were divided into three groups injected with different agents via lingua vein control group (animals received equivalent saline) , LPS group (animals received a bolus dose of LPS 5 mg?0.5 mL-1?kg-1) and LPS+ curcumin group (animals received AP- 1 inhibitor curcumin 20 mg?0.5 mL-1?kg-120 min before the injection of LPS 5 mg ?0.5 mL-1?kg-1) . The expression of HO-1 mRNA and protein in the lung were examined 7 h after LPS administration by reverse transcribed polymerase chain reaction (RT- PCR) and Western blotting, respectively. Carboxyhemoglobin (HbCO) formation within pulmonary tissue was measured to represent CO content.RESULTS:
The results showed that HO- 1 mRNA and protein expression as well as CO content in the lung of rats in LPS group were significantly higher than those in control group (P
Full text:
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Index:
WPRIM
Language:
Zh
Journal:
Chinese Journal of Pathophysiology
Year:
2000
Type:
Article