Effect of alive lactobacillus rhamnosus GG (LGG) on IL-8 production in Caco-2 cells induced by TNF-? / 中华围产医学杂志

ABSTRACT

Objective To test the influence of Lactobacillus rhamnosus GG (LGG) in down-regulating the IL-8 production in Caco-2 cells induced by TNF-?. Methods Caco-2 cells were treated with living LGG (1?106 CFU/L) with or without the presence of TNF-? in the culture media. The production of IL-8 was measured by quantitative ELISA. Results TNF-? significantly increased IL-8 secretion by Caco-2 cells at 24 h and 48 h of incubation as compared with the control group (5.33 ng/mg vs 7.36 ng/mg, 15.69 ng/mg vs 32.29 ng/mg, P