Determination of Resveratrol in Serum and Organs of Mice by RP-HPLC / 中国药房
China Pharmacy
; (12)2005.
Article
in Zh
| WPRIM
| ID: wpr-528470
Responsible library:
WPRO
ABSTRACT
OBJECTIVE:
To develop RP-HPLC method for the rapid determination of resveratrol in serum and tissue homogenates (in some organs) of mice.METHODS:
HPLC determination was performed using Kromasil C18 column,the mobile phase consisted of methanol - water (48∶ 52) with a flow rate of 1ml/min,the column was under room temperature with its pressure at 12kPa,the detection wavelength was 303nm and the sample size was 20?l.RESULTS:
The linear range for resveratrol was 0.25~25?g/ml, with minimal detectable drug concentration at 0.1?g/ml (S/N=3). The average absolute rec_overy of the samples was above 88% and the methodological recovery rates of which ranged from 96% to 100%,RSD were below 10%.CONCLUSIONS:
The established determination method is economical, reliable, simple and rapid, and suitable for the blood concentration determination of resveratrol and the study of its distribution in animal body.
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Index:
WPRIM
Language:
Zh
Journal:
China Pharmacy
Year:
2005
Type:
Article