Expression of muclear factor-kappa B in hepatocellular carcinoma tissue and its significance / 中国普通外科杂志

ABSTRACT

Objective To investigate the gene expression of nuclear factor-kappa B(NF-?B) and its (biological) significance in liver cancer tissue.Methods The samples from hepatic cancer tissue and(corresponding) adjacent tissues were collected in thirty-two patients with primary liver cancer.The mRNA and protein levels of NF-?B were determined by reverse-transcription PCR and Western bolt,respectively.(Immunochemistry) was employed to determine the localization of NF-?B protein in liver tissues.Results As shown by RT-PCR and Western blot,both mRNA and protein expressions of NF-?B in liver cancer tissues were significantly higher than those in corresponding adjacent liver tissues(P