Effect of propolis water extract on apoptosis of human umbilical vein endothelial cells in vitro / 中国病理生理杂志

ABSTRACT

AIM:To explore the effect of water extract propolis(WEP)from Taishan on apoptosis of human umbilical vascular endothelial cells(HUVECs)induced in vitro by tumor necrosis factor-?(TNF-?).METHODS:HUVECs were collect by digestion-perfusion and cultivated.TNF-? at concentration of 50 ?g/L was administrated to induce the apoptosis of HUVECs.After injury,HUVECs were treated with WEP at concentrations of 50,100,and 200 mg/L,respectively,for 24 h.Apoptosis was evaluated by terminal-deoxynucleotidyl transferase mediated nick end labeling(TUNEL)and flow cytometry(FCM).RESULTS:Apoptosis index in injured group was significantly higher than that in control group,and decreased significantly after treating with WEP at concentrations of 50,100,and 200 mg/L,respectively(P