Effects of adiponectin siRNA on the glucose transport in 3T3-L1 adipocytes / 中国病理生理杂志
Chinese Journal of Pathophysiology
; (12)2000.
Article
in Zh
| WPRIM
| ID: wpr-531399
Responsible library:
WPRO
ABSTRACT
AIM:
To construct the adenovirus vector with adiponectin (Acrp30) siRNA, and to observe its effect on the Acrp30 expression and glucose transport in 3T3-L1 adipocytes.METHODS:
Mouse Acrp30 siRNA fragment was designed, synthesized and cloned into the adenovirus vector. 3T3-L1 cells were infected with the two recombinant adenoviruses, respectively. The mRNA expression and protein levels of Acrp30 in these cells were evaluated by RT-PCR and ELISA. Glucose transport was measured by 2-Deoxy-[3H]-D-glucose incorporation method.RESULTS:
The recombinant adenoviruses were successfully constructed. They remarkably downregulated the expression of Acrp30 at both mRNA and protein levels in 3T3-L1 cells, and decreased the glucose transport in 3T3-L1 adipocytes (P
Full text:
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Index:
WPRIM
Language:
Zh
Journal:
Chinese Journal of Pathophysiology
Year:
2000
Type:
Article