Metabolic loading of guanosine induces chondrocyte apoptosis via the Fas pathway
Experimental & Molecular Medicine
; : 401-407, 2006.
Article
in En
| WPRIM
| ID: wpr-53150
Responsible library:
WPRO
ABSTRACT
Although the apoptosis of chondrocytes plays an important role in endochondral ossification, its mechanism has not been elucidated. In this study, we show that guanosine induces chondrocyte apoptosis based on the results of acridine orange/ ethidium bromide staining, caspase-3 activation, and sub-G1 fraction analysis. The potent inhibitory effect of dipyridamole, a nucleoside transporter blocker, indicates that extracellular guanosine must enter the chondrocytes to induce apoptosis. We found that guanosine promotes Fas-Fas ligand interaction which, in turn, leads to chondrocyte apoptosis. These findings indicate a novel mechanism for endochondral ossification via metabolic regulation.
Key words
Full text:
1
Index:
WPRIM
Main subject:
Membrane Glycoproteins
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Signal Transduction
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Rats, Sprague-Dawley
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Apoptosis
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Receptors, Tumor Necrosis Factor
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Fas Receptor
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Chondrocytes
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Nucleoside Transport Proteins
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Tumor Necrosis Factors
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Fas Ligand Protein
Limits:
Animals
Language:
En
Journal:
Experimental & Molecular Medicine
Year:
2006
Type:
Article