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Determination of Concentration of Netilmicin Sulfate in Human Serum by Ion-pair RP-HPLC / 中国药房
China Pharmacy ; (12)2007.
Article in Zh | WPRIM | ID: wpr-533259
Responsible library: WPRO
ABSTRACT
OBJECTIVE:To establish an ion-pair RP-HPLC method for determination of the concentration of netilmicin sulfate in human serum.METHODS:The determination was performed on Luna C18 column with column temperature set at 40℃;the mobile phase consisted of acetonitrile-water (15∶85,pH 2.0,containing 10 mmol?L-1 sodium heptanesulfonate) at a flow rate of 0.8 mL?min-1.The detection wavelength was set at 205 nm.RESULTS:The linear range of netilmicin was 2~20 mg?L-1(r=0.997 6) with a recovery rate of 93.66%~96.05%.The intra-day and inter-day RSD were both less than 10%,respectively.CONCLUSION:The method is sensitive and simple with accurate and reliable results,and it is able to satisfy the requirement for the determination of serum concentration of netilmicin.
Key words
Full text: 1 Index: WPRIM Language: Zh Journal: China Pharmacy Year: 2007 Type: Article
Full text: 1 Index: WPRIM Language: Zh Journal: China Pharmacy Year: 2007 Type: Article