A bioassay method for human Granulocyte—Macrophage Colony stimulating factor with DMSO—induced HL60 cell line / 中国免疫学杂志

ABSTRACT

Based on the high level of human granulocyte—macrophage colony—stimulating factor(HGM—CSF)re-ceptor of HL60 cell line upon induction by DMSO,we developed a hioassay method for HGM—CSF with ahigher specificity and sensitivity.The sensitivity of this assay reached to 0.3ng/ml by selecting the optimalHL60 cell—induced time,cell density and GM—CSF—stimulated time.With this assay,we are able to quantifythe level of rHuGM—CSF as well as conditioned media from human bladder carcinoma cell line U5637 screatinga high level of GM—CSF.