Biological characteristics of stromal cells derived from rat bone marrow in vitro / 实用口腔医学杂志
Journal of Practical Stomatology
;
(6)1996.
Article
in Chinese
| WPRIM
| ID: wpr-535790
ABSTRACT
砄bjectiveTo study the biological characteristics and mineralization activity of strom cells (MSC) isolated from rat bone marrow.Methods:
MSCs were isolated from rat bone marrow using gradient centrifuge (460 g,20 min) with 70% percoll and cultured with DMEM. The cells were then incubated with mineralization culture medium (MCM) in the presence of Dex (10~7 mol/L), ? GP (10 mmol/L) and AcA (50 g/L).Cell growth was studied with cell counting,alkaline phosphatase (ALP) expression with a kit and mineralization with a saturated solution of alizarin red S (pH 4.3) stainning.Results:
The isolated cells from bone marrow of rat were epithelial like. The doubling time (DT) of the cells in MCM or DMEM were 50.3 h and 66.1 h respectively. Mineralization was observed after the cells had been cultured in MCM for 28 days. bFGF and rhBMP 2 increased ALP expression of MSC in a dosage dependent manner.Conclusion:
In vitro cultured MSCs may be induced to differentiate towards osteoblasts.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Journal of Practical Stomatology
Year:
1996
Type:
Article
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