The effect of Trichosanthin on cytokines production and ion channel of human PB-MC / 中国免疫学杂志
Chinese Journal of Immunology
; (12)1999.
Article
in Zh
| WPRIM
| ID: wpr-536360
Responsible library:
WPRO
ABSTRACT
Abstract Objective:
To study the effect of Trichosanthin on cytokines production and ion channel of human PBMC, to demonstrate its immune regulatory mechanism.Methods:
The levels of IL-2 and IL-6 produced by PBMC under stimulation of PHA-M were measured by radio-immunoassay after cultured with Trichosanthin. The current intensity of voltage-dependent potassium channel [K(V) channel] were recorded by patch-clamp technique after added Trichosanthin.Results:
Both IL-2 and IL-6 production were enhanced by Trichosanthin, and the current in-tensity of K(v) channel were markedly increased from (210.2 ?53.6)PA to (2 223.0 ?310.7)PA.Conclusion:
Trichosanthin can enhance the production of both IL-2 and IL-6 of human PBMC and increase the current intensity of K( v) channel on T lymphocyte membrane, which im-prove cell-mediated immunity and humoral immunity.
Full text:
1
Index:
WPRIM
Language:
Zh
Journal:
Chinese Journal of Immunology
Year:
1999
Type:
Article