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Identification of Proteins Differentially Expressed in the Conventional Renal Cell Carcinoma by Proteomic Analysis
Article in En | WPRIM | ID: wpr-53828
Responsible library: WPRO
ABSTRACT
Renal cell carcinoma (RCC) is one of the most malignant tumors in urology, and due to its insidious onset patients frequently have advanced disease at the time of clinical presentation. Thus, early detection is crucial in management of RCC. To identify tumor specific proteins of RCC, we employed proteomic analysis. We prepared proteins from conventional RCC and the corresponding normal kidney tissues from seven patients with conventional RCC. The expression of proteins was determined by silver stain after two-dimensional polyacrylamide gel electrophoresis (2D-PAGE). The overall protein expression patterns in the RCC and the normal kidney tissues were quite similar except some areas. Of 66 differentially expressed protein spots (p<0.05 by Student t-test), 8 different proteins from 11 spots were identified by MALDI-TOF-MS. The expression of the following proteins was repressed (p<0.05); aminoacylase-1, enoyl-CoA hydratase, aldehyde reductase, tropomyosin alpha-4 chain, agmatinase and ketohexokinase. Two proteins, vimentin and alpha-1 antitrypsin precursor, were dominantly expressed in RCC (p<0.05).
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Full text: 1 Index: WPRIM Main subject: Tropomyosin / Ureohydrolases / Vimentin / Comparative Study / Carcinoma, Renal Cell / Electrophoresis, Gel, Two-Dimensional / Alpha 1-Antitrypsin / Aldehyde Reductase / Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Proteome Type of study: Diagnostic_studies / Screening_studies Limits: Aged / Female / Humans / Male Language: En Journal: Journal of Korean Medical Science Year: 2005 Type: Article
Full text: 1 Index: WPRIM Main subject: Tropomyosin / Ureohydrolases / Vimentin / Comparative Study / Carcinoma, Renal Cell / Electrophoresis, Gel, Two-Dimensional / Alpha 1-Antitrypsin / Aldehyde Reductase / Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Proteome Type of study: Diagnostic_studies / Screening_studies Limits: Aged / Female / Humans / Male Language: En Journal: Journal of Korean Medical Science Year: 2005 Type: Article