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Amplification of SAG3 gene fragment and its application in detection of toxoplasma gondii in hemato- poietic stem cell transplantation / 中华器官移植杂志
Article in Zh | WPRIM | ID: wpr-539551
Responsible library: WPRO
ABSTRACT
Objective To establish a rapid, specific and sensitive diagnostic technique for the human T. gondii infection with hematopoietic stem cell transplantation and discuss its clinical significance. Me- thods Fifty-six patients subject to hematopoietic stem cell transplantation were detected with ELISA and PCR. Results Among 56 recipients of hematopoietic stem cell transplantation, 7 were positive for T. gondii antigen and 10 were positive for SAG3 gene fragment respectively with the positive rate being 14.3 % and 17.8 % in the ELISA and PCR screening respectively. Twenty healthy people were negative for anti-Toxo antibody.Conclusion PCR is an accurate, relatively rapid, sensitive and specific method for detecting SAG3 gene of T. gondii, and can be considered a valuable additional tool for identification of T. gondii infections.
Key words
Full text: 1 Index: WPRIM Type of study: Diagnostic_studies Language: Zh Journal: Chinese Journal of Organ Transplantation Year: 1996 Type: Article
Full text: 1 Index: WPRIM Type of study: Diagnostic_studies Language: Zh Journal: Chinese Journal of Organ Transplantation Year: 1996 Type: Article