Study of E-cadherin Antisense Oligodeoxynucleotide on Invasive Ability of Tumor Cells / 中国普外基础与临床杂志

ABSTRACT

Objective To study the effect of down-regulation of E-cadherin on the invasion ability of tumor cells.Methods Human pancreatic carcinoma cell line JHP-1 was treated with E-cadherin antisense oligodeoxynucleotied(ASODN).The immunocytochemistry,Western blot were used to detect the expression and the contents of(E-cadherin) in the tumor cells,and the invasive ability of tumor cells were evaluated by invasive-MTT assay.Results Treated with E-cadherin ASODN,the expression of E-cadherin on JHP-1 cells were reduced,and the protein contents were decreased as well compared with control groups and ODN group.The invasive ability of JHP-1 cells to the basement membrane was increased(P