Procaryotic expression of human FceR I a subunit extracellular domain and its binding mechanism with IgE / 第二军医大学学报
Article
in Zh
| WPRIM
| ID: wpr-554451
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WPRO
ABSTRACT
Human FceR I a subunit extracellular domain was cloned and expressed with 2 different expressing systems and Dot blot was used to detect its biological activity to bind with IgE,providing reference for binding mechanism of human FceR I a subunit extracellular domain with IgE. It was shown that FceR I a subunit extracellular domain from pBAD/g I A expressing system could bind with IgE, but the one from PQE30 expressing system could not bind with IgE. It is suggested that FceR I a subunit extracellular domain alone is sufficient to bind with IgE without P and Y subunit. The proper space configuration and disulfide bond of FceR I a subunit is necessary for binding with IgE, but its glycosylation is unnecessary.
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Language:
Zh
Journal:
Academic Journal of Second Military Medical University
Year:
2000
Type:
Article