Rapid detection of Amp C ?-Iactamase in Enterobacter cloacae by PCR / 第二军医大学学报
Academic Journal of Second Military Medical University
;
(12)1999.
Article
in Chinese
| WPRIM
| ID: wpr-555858
ABSTRACT
Objective:
To establish a new method to detect Amp C?-lactamase by PCR.Methods:
The amp C and amp D gene fragments of E. cloacae were amplified by the amp C and amp D primers to detect Amp C ?-lactamase, especially the enduring and highly productive enzymes.Results:
Totally 193 of 214 strains of E. cloacae were positive for amp C gene , implicating most strains of E. cloacae had the ability to produce the enzyme. Sixteen of the 193 strains (amp C positive) were negative for amp D genes, implicating these 16 strains could produce the enduring and highly productive enzymes. The results were confirmed by cefoxitin three-dimensional test.Conclusion:
The new method to detect Amp C ?-lactamase, especially the enduring and highly productive enzymes,by amp C and amp D primers is a rapid and accurate method.
Full text:
Available
Index:
WPRIM (Western Pacific)
Type of study:
Diagnostic study
Language:
Chinese
Journal:
Academic Journal of Second Military Medical University
Year:
1999
Type:
Article
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