Identification of Candida albicans genes with cDNA technique from LongSAGE tags / 第三军医大学学报

ABSTRACT

Objective To identify the LongSAGE tags from yeast LongSAGE library and hyphal LongSAGE library of Candida albicans. Methods The technique called the generation of longer cDNA fragments from serial analysis of gene expression (SAGE) tags was used for gene identification (GLGI). The SAGE tags of 17 bases were converted into their corresponding 3′ cDNA fragments covering hundred bases, and the sequence was analyzed. Results Fifteen out of twenty tags were stably and efficiently extended with the base size between 200-1 000 bp. The sequence was considered to represent a known gene since it matched a full-length transcript sequence with over 95% similarity in the same orientation, including the same 17-bp SAGE tag sequence. Conclusion By using novel SAGE tags as primer, we can identify efficiently many novel transcripts. A combined application of SAGE-GLGI can be used to define the boundary of expressed genes in the genomic sequences in Candida albicans and in other eukaryotic genomes.