Nerve regeneration factor promotes nerve regeneration after sciatic nerve injury in rats / 第二军医大学学报

ABSTRACT

Objective:To investigate the promoting effect of nerve regeneration factor(NRF) on nerve regeneration after sciatic nerve injury in rats.Methods: Thirty Sprague-Dawely rats,including 15 females and 15 males,were equally randomized into 3 groups low dose NRF group,high dose NRF group and control group.Rats' sciatic nerves were injured by crushing and sciatic function index(SFI) was determined by walking tract analysis at days 10,15 and 20 after crushing.Then sciatic nerves on both sides were dissociated for electrophysiology study and the recovery rate of nerve conduction velocity(NCV) was calculated.Then,2 rats were randomly selected from each group and the ultrastructure of regenerated sciatic nerves was observed by electron microscope.Meanwhile,the spinal cord segments(L_4-L_6),the distal site of injured sciatic nerve and the injured gastrocnemius muscle in other rats were observed by light microscope.The count of motor neurons of anterior horn,the number of myelinated fibers,the transverse section area of gastrocnemius muscle cells and other parameters were determined.Results: At day 10 after crushing,SFI scores had no significant difference between 3 groups;at day 15 after crushing,SFI score of high dose NRF group was significantly higher than that of the control group(P