Morphological observation of embryonic stem cell-derived neurons after transplanted into A?-injured rat hippocampus / 第三军医大学学报
Journal of Third Military Medical University
;
(24)1988.
Article
in Chinese
| WPRIM
| ID: wpr-563241
ABSTRACT
Objective To observe the morphology of embryonic stem cell-derived neurons after transplanted into A?-injured rat hippocampus. Methods Neural precursor cells (NPCs) were generated from mouse embryonic stem cells (ESCs) expressing EGFP with modified serum-free methods and then transplanted into the hippocampus of A?1-40-injuried rats. The morphology, neurotransmitter phenotypes and receptors of EGFP-positive donor cells were observed with immunofluorescene methods. Results The engrafted NPCs survived and differentiated into glutamatergic and GABAergic neurons and expressed both glutamatergic and GABAergic neurotransmitter receptors. Synaptophysin-positive dots were found surrounding somata and dendrites of transplanted neurons, suggesting the presence of presynaptic terminals adjacent to their membranes. Conclusion NPCs derived from ESCs can differentiate into excitatory and inhibitory neurons after grafted into Alzheimer's disease model rats, and maybe form synapse with the host neurons.
Full text:
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Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Journal of Third Military Medical University
Year:
1988
Type:
Article
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