Identification and analysis of effective compositions of Schistosoma japonicum 31-32 kDa proteins / 中国血吸虫病防治杂志
Chinese Journal of Schistosomiasis Control
; (6)1989.
Article
in Zh
| WPRIM
| ID: wpr-563637
Responsible library:
WPRO
ABSTRACT
Objective To identify and analyze the effective compositions of Schistosoma japonicum 31-32 kDa proteins by using the techniques of proteomics.Methods The total proteins were prepared from 32-day adult worms of Schistosoma japonicum.After two-dimensional(2-D)gel electrophoresis,the distinct protein spots from 2-D gels were isolated and analyzed by MALDI-TOF-MS.Results A total of 13 protein spots,within the range of 31-32 kDa,were detected in the 2-D gels.Three of them had high homology with Actine-2 of S.mansoni,glycerol-3-phosphate dehydrogenase of S.japonicum and cathepsin B endopeptidase of S.mansoni.Conclusions The 31-32 kDa antigens contain 3 important antigensactine-2,glycerol-3-phosphate dehydrogenase and cathepsin B endopeptidase,which have been demonstrated to have certain protective effect against S.japonicum.Our findings can facilitate the development of multi-epitope vaccine against S.japonicum.
Full text:
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Index:
WPRIM
Type of study:
Diagnostic_studies
/
Prognostic_studies
Language:
Zh
Journal:
Chinese Journal of Schistosomiasis Control
Year:
1989
Type:
Article