Analysis and quantitative determination of fatty acids in Hericium Erinaceus polysaccharide / 中成药
Chinese Traditional Patent Medicine
; (12)1992.
Article
in Zh
| WPRIM
| ID: wpr-574198
Responsible library:
WPRO
ABSTRACT
AIM:
To assay fatty acids in Hericium Erinaceus polysaccharide by GC for establishing a method for quantitative determination of fatty acids.METHODS:
The kinds of fatty acids were detected by GC-MS. The fatty acids contents were determined by GC The sample was dissolved in sulphuric acid-methanol (1∶20) containing 0.5 mg heptadecanoic acid methyl ester and kept at 80?C for 3 hr. Then, the solution was extracted by hexamethylene four times and diluted to 1 mL. The extraction (0.4 ?L) was injected on a DB-1 column, using nitrogen as the carrier gas. The temperature was raised from 60?C to 200?C at the rate of 10?C?min -1 , and subsequently sustained for 30 min. The injection port and detector temperature were both at 250?C.RESULTS:
The fatty acids were separated completely. The linear ranges were 0.4-3.6 mg?mL -1 . RSD97%.CONCLUSION:
The fatty acids can be determined accurately by this method.
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Index:
WPRIM
Language:
Zh
Journal:
Chinese Traditional Patent Medicine
Year:
1992
Type:
Article