Study on hematopoietic reconstruction for bone marrow depression mice with the transplantation of mobilized peripheral blood stem/proginiter cells by ginseng polysaccharide / 重庆医科大学学报
Journal of Chongqing Medical University
;
(12)2003.
Article
in Chinese
| WPRIM
| ID: wpr-576171
ABSTRACT
Objective:
To study the hematopoietic reconstruction for bone marrow depression mice with mobilized peripheral blood stem/proginiter cells by ginseng polysaccharide.Methods:
Establish hematopoiesis failure model of mouse and transplant murine peripheral blood mononuclear cells mobilized by GPS.To observe the survival time,WBC,the weight of spleen,the number of CFU-S of recipient mice and chromosome chimerism.Results:
After PBMNCs(1?105)mobilized by GPS from mice(male) were injected to the lethality irradiated mice,it can reduce the time of WBC recovery obviously;prolong the time of murine survival,increase the number of WBC of the recipient mice;it can also increase the number of CFU-S,the weight of spleen;Evaluating the Y chromosome by PCR,the result showed that 581bp Y chromosome sequence was examined in peripheral blood of the recipient mice.Conclusion:
There are a lot of hematopoietic stem/ proginiter cells in peripheral blood of the mice mobilized by GPS,so it can obviously reconstruct the hematopoiesis of the bone marrow depression mice by transplantation.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Journal of Chongqing Medical University
Year:
2003
Type:
Article
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