Influence of intestinal flora dysregulation induced by antibiotic on immunity and toll-like receptor 2,4 gene expression in mice / 重庆医科大学学报

ABSTRACT

Objective:To study the effects of intestinal flora dysregulation induced by antibiotic on immune function and toll-like receptor(TLR)2,4.Methods:Sixty BALB/c mice were divided into two groupsantibiotic and control group.The mice in antibiotic group were treated with cefoperazone orally for consecutive 7 days.After 7 days,mice were inoculated with Candida albicans.Results:The quantity of Enterobacteriaceae,Enterococcus,Bifidobacterium and Lactobacillus in antibiotic therapy group was significantly lower than that in the control group.The quantity of Candida albicans increased in the antibiotic therapy group.Spleen index,lymphocytic transformation rate induced by PHA,delayed-type hypersensitivity and the number of antibody secreting cells,the gene expression of TLR2 and TLR4 mRNA in spleen were lower than those in the control group.Conclusions:The intestinal flora dysregulation induced by antibiotic may decrease the immune function and toll-like receptor 2,4 gene expression in mice.