Advanced glycation end-products decreases THP-1 macrophages expression of peroxisome proliferator activated receptor-? / 重庆医科大学学报

ABSTRACT

Objective:To investigate the effect of advanced glycosylation end-products(AGEs) on the expression of peroxisome proliferator-activated receptor-?(PPAR?) in THP-1 macrophages and explore the function of AGEs in atherosclerosis.Methods: Differentiation of THP-1 cell was induced by PMA.The THP-1 macrophages were exposed to AGE-modified bovine serum albumin(AGE-BSA) in different concentration or the same concentration for different time.Expression of PPAR? mRNA and protein in THP-1 macrophages was measured by RT-PCR and immunocytochemical method.Results:RT-PCR showed that expression of PPAR? mRNA was 1.235?0.044,0.752?0.055,0.494?0.026,0.277?0.025 in 50,100,200 and 400?g/ml AGE-BSA groups.PPAR? mRNA expression in THP-1 macrophages following 200?g/ml AGE-BSA for 12,24,36,48h was 1.260?0.043,0.467?0.033,0.360?0.012.Western-Blot showed that after exposure of THP-1 macrophages to 50,100,200and 400mg/L AGE-BSA,the average integrated optical density values of PPAR? protein expression were 36.460?0.625,24.561?0.636,19.326?0.803,12.715?0.752 respetively,significantly lower than that in BSA group(P