Determination of serum?-fetoprotein by TRFIA,RIA and ELISA / 重庆医科大学学报
Journal of Chongqing Medical University
;
(12)2003.
Article
in Chinese
| WPRIM
| ID: wpr-578156
ABSTRACT
Objective:
To assess the efficacy of time-resolved fluoroimmuno assay(TRFIA) for quantative determination of?-fetoprotein(AFP) in comparison with radio immuno assay(RIA) and enzme linked immunoabsorbent assay(ELISA).Methods:
Thirty serum samples from randomly selected patients were collected to test AFP content with TRFIA,RIA and ELISA respectively.Results:
The linear range of AFP was 1 to l 000 ng/ml for TRFIA,5~400ng/ml for RIA and 5~300ng/ml for ELISA.The intraassay coefficients of variation(CV) and interassay CV were 3.3% and 4.7% for TRFIA,5.3% and 8.9% for RIA,and 10.5% and 15.2% for ELISA,respectively.Correlation tests revealed that the results of RIA were more closely correlated with those of TRFIA than with those of ELISA.Conclusion:
TRFIA is shown to be a better method for AFP quantitation than RIA and ELISA in terms of precision and linear range.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Journal of Chongqing Medical University
Year:
2003
Type:
Article
Similar
MEDLINE
...
LILACS
LIS