Determination of Naringin in Fructus Ponciri Trifoliatae Immaturi Collected in Different Seasons / 中国中医药信息杂志

ABSTRACT

Objective To study the best collected time of fructus ponciri trifoliatae determine the naringin in it by RP-HPLC method. Methods The hypersil ODS C18 (250 mm?4.6 mm, 25 ?m) column was used. The mobile phase consisted of acetonitrile-0.1% phosphoric acid (21∶79), the detection wavelength was 283 nm. The flow rate was 1.0 mL/min. Result The calibration curve of naringin was in good linearity over 0.164 4～0.832 0 ?g, and the regression equation was Y =156 681X -84 834 (r =0.999 9). The average recovery was 98.49%, and RSD=2.68%. Conclusions The method is simple, quick, sensitive, accurate, reproducible and can be used to control the quality. Fructus ponciri trifoliatae should be collected in the beginning and middle of June.