Determination of Water Soluble Components in Salvia miltiorrhiza Bge. by HPLC / 中国中医药信息杂志

ABSTRACT

Objective To establish a method for determination of the water-soluble components of Salvianolic acid B,Danshensu and Protocatechuic aldehyde in Salvia miltiorrhiza Bge. Method Chromatography was performed on Kromosil C18 (4.6 mm?250 mm,5 ?m). Mobile phase Ⅰ was methanol-acetonitrile-formicacid-water (30∶10∶1∶59),mobile phase Ⅱ was methanol-acetic acid-water (20∶1∶80),the flow rate was 1.0 mL/min,the detection wavelength was set at 286 and 280 nm,column temperature was 35 ℃. Result Determined 10 different habitats Salvia miltiorrhiza Bge.,the average content of Salvianolic acid B was 0.09%～6.81%,the average recovery was 99.64%,RSD=1.09%. The average content of Danshensu was 0.16%～0.68%,the average recovery was 98.94%,RSD=1.49%. The average content of Protocatechuic aldehyde was 0.01%～0.09%,the average recovery was 98.96%,RSD= 1.66%. Conclusion The method is accurate and reliable with good reproducibility,and can be used as determination standard for Salvia miltiorrhiza Bge.