Content Determination of Hesperidin in Shuhouyin by HPLC / 中国中医药信息杂志
Chinese Journal of Information on Traditional Chinese Medicine
;
(12)2006.
Article
in Chinese
| WPRIM
| ID: wpr-578819
ABSTRACT
Objective To establish a HPLC method for the content determination of hesperidin in Shuhouyin. Methods Chromatographic assay was performed on CenturySIL C18-AQ (250 mm?4.6 mm, 5 ?m) column at room temperature. The mobile phase was acetonitrile-0.2% phosphoric acid solution (21∶79). The flow rate was 0.8 mL/min. The detection wavelength was at 284 nm. The sample size was 20 ?L. Results Good linear relationship of hesperidin achieved in the range of 1.004~10.04 ?g. The average recovery rate was 98.59% with RSD=0.72%. Conclusion The method is simple, accurate and stable with good reproducibility. This method can be used for determination of hesperidin in Shuhouyin.
Full text:
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Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Information on Traditional Chinese Medicine
Year:
2006
Type:
Article
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