Establishment and Identification for Macrophage-derived Foam Cell Model of THP-1 Cell Line / 中药新药与临床药理

ABSTRACT

Objective To establish a macrophage-derived foam cell model of human monocytic THP-1 cell line and to identify the foam cell.Methods THP-1 cells were differentiated into macrophages after induction with 160 nmol/L phorbol-1-myristate-13-acetate for 24 hours and then incubation with 80 mg/L oxidized low density lipoprotein(oxLDL) for 48 hours.The differentiated cells were observed after oil red O staining under light microscope.High performance liquid chromatography/atmospheric pressure chemical ionization tandem mass spectrometry(HPLC-MS) was used for quantitative analysis of cellular cholesterol and cholesteryl esters contents.Results There was a large number of red lipid droplets in phorbol-1-myristate-13-acetate and oxLDL treated THP-1 cells after oil red O staining under light microscope.Cellular contents of cholesterol and cholesteryl esters in the differentiated cells increased markedly compared with the normal THP-1 monocytes by HPLC-MS assay.Conclusion A human monocyte-derived foam cell model has been established by incubating THP-1 cells with 120nmol/L phorbol-1-myristate-13-acetate for 24 and then with 80 mg/L oxidized low density lipoprotein for 48 hours.