Determination of narigin,hesperidin and neohesperidin in Weili Tablet by HPLC / 中成药

ABSTRACT

AIM: To develop an HPLC method for determining narigin,hesperidin and neohesperidin in Weili Tablet(Rhizoma Pinelliae,Radix et Rhizoma Gentianae,Fructus Aurantii immaturus etc. ). METHODS: The analysis was carried out with a Diamonsil C18(4. 6 mm ? 250 mm,5 ?m)column and the mobile phase of acetoni-trile-1% glacical acetic acid solution(19:81,v/v). The flow rate was 1. 0 mL/min and column temperature was 30 ℃. The detection wavelength was set at 283 nm. RESULTS: The calibration curve was linear within the range of 6. 0-60. 0 ?g/mL,2. 24-22. 4 ?g/mL and 10. 2-102. 0 ?g/mL for narigin,hesperidin and neohesperidin,respec-tively. The average recoveries were 100. 2% ,98. 8% and 97. 1% for narigin,hesperidine and neohesperidine,re-spectively. CONCLUSION: The method is reproducible,sensitive and accurate for determination of narigin,hes-peridin and neohesperidin in Weili Tablet.