Effect of simulated CO_2 pneumoperitoneum on cell proliferation of human renal carcinoma 786-O cells line / 重庆医科大学学报

ABSTRACT

Objective:Tostudythe effects ofsimulated carbon dioxide pneumoperiton eumenvironment on cell proliferation of human renal carcinoma 786-O cells in vitro.Methods:The renal carcinoma 786-O cells were exposed to simulated CO2 pneumoperitoneum environment for different time periods(2 h or 4 h)under the pressure of 14 mmHg and those cultured in normal experimental condition as control,then proliferation viability of 786-O cells was observed by MTT assay close behind the exposure;cell cycle and apoptotic rate were detected by flow cytometry,the protein expression of PCNA and Survivin were examined by immunocytochemistry 48 h after exposure respectively.Results:Compared with the control group,Both of the treated groups showed cell proliferation was significantly increased (P