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Effect of simulated CO_2 pneumoperitoneum on cell proliferation of human renal carcinoma 786-O cells line / 重庆医科大学学报
Article in Chinese | WPRIM | ID: wpr-581308
Responsible library: WPRO
ABSTRACT

Objective:

Tostudythe effects ofsimulated carbon dioxide pneumoperiton eumenvironment on cell proliferation of human renal carcinoma 786-O cells in vitro.

Methods:

The renal carcinoma 786-O cells were exposed to simulated CO2 pneumoperitoneum environment for different time periods(2 h or 4 h)under the pressure of 14 mmHg and those cultured in normal experimental condition as control,then proliferation viability of 786-O cells was observed by MTT assay close behind the exposure;cell cycle and apoptotic rate were detected by flow cytometry,the protein expression of PCNA and Survivin were examined by immunocytochemistry 48 h after exposure respectively.

Results:

Compared with the control group,Both of the treated groups showed cell proliferation was significantly increased (P

Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Journal of Chongqing Medical University Year: 2007 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Journal of Chongqing Medical University Year: 2007 Type: Article