The immunoprecipitation method for the determination of ?-glutamyltransferase complexed to two lipoproteins in serum and its application for the diagnosis of liver malignancies / 中华检验医学杂志
Chinese Journal of Laboratory Medicine
; (12)2000.
Article
in Zh
| WPRIM
| ID: wpr-583092
Responsible library:
WPRO
ABSTRACT
Objective To establish the immunoprecipitation method to detect the activity of ?-glutamyltransferase (GGT) complexed to low-density-lipoprotein and very-low-density-lipoprotein in serum (LDL-LDL-GGT) and to observe the diagnostic efficiency in discriminating liver malignancies from other hepatopathy diseases.Methods Using apo B antibody as a precipitant to precipitate the parts of GGT which connected to LDL and VLDL. 65 cases of liver malignancies, 53 cases of cirrhosis, 32 of chronic hepatitis and 75 of health people were analysed. Results Apo B antibody can precipitate LDL and VLDL thoroughly with various serum samples. The withinrun CV are 3 6%~8 2% and the betweenrun CV are 5 5%~9 8%. The linearity range is 0~587 U/L. With the cut off value of 10 U/L, the sensitivity for liver malignancies is 86 2%, the specificities for discriminating liver malignancies from cirrhosis and chronic hepatitis is 67 9% and 81 3% respectively. Conclusion It has important significance for the diagnosis of liver malignancies with the immunoprecipitation method for determination of serum LDL-VLDL-GGT activity.
Full text:
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Index:
WPRIM
Type of study:
Diagnostic_studies
Language:
Zh
Journal:
Chinese Journal of Laboratory Medicine
Year:
2000
Type:
Article