Therapeutic effect of recombinant angiogenic inhibitor CHM- I on osteosarcoma / 中国肿瘤生物治疗杂志

ABSTRACT

Objective: To study the effects of recombinant human Chondromodulin-I protein in treatment of osteosarcoma. Methods: Human Chondromodulin-I gene was amplified from cartilage tissue by RT-PCH and was cloned into pro-caryotic expression vector Pet28a( + ). The recombinant plasmid Pet( CHM-I) was transformed into E. coll BL21 ( DE3 ) ; the product was fused with 6 ?His at N-terminal and was purified by Ni2 +2NTA ion exchange resin. The formation of tube-like cellular networks was assayed by co-incubation of HUVECs and recombinant human (Chondromodulin-I. The proliferation of the cells incubated with the recombinant human Chondromodulin-I protein was examined by MTT assay and cell number counter Null mice received local injection of recombinant human Chondromodulin-I protein into the tumors. Results: Rcombinant human CHM-I protein was successfully obtained. The purified CHM-I did not inhibit the proliferation of tumour cells,but inhibited the formation of tubelike cellar network in vitro and tumor growth. Conclusion:Recom-bitant human Chondromodulin-I protein can inhibit angiogenesis in vitro and growth of tumor in vivo.