Quantitative detection of Rabies virus antibody by chemiluminescent immunoassay / 临床检验杂志
Chinese Journal of Clinical Laboratory Science
; (12)2006.
Article
in Zh
| WPRIM
| ID: wpr-586126
Responsible library:
WPRO
ABSTRACT
Objective To establish chemiluminescent immunoassay(CLIA) for quantitative detection of Rabies virus antibody in human serum.Method The purified antigen was coated on the microplate,pairing with alkaline phosphatase(ALP)-labeled antigen and luminescence substrate CSPD,then the double antigen sandwich CLIA was established.Results The sensitivity of the assay was 0.2IU/ml.The linear range was from 0 IU/ml to 200 IU/ml.The precision was less than 10%.The analytical recovery rate was from 90% to 110%.Conclusion The CLIA is a simple,sensitive,special and repeatable method for detection of Rabies virus antibody.It was suitable for clinical application.
Full text:
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Index:
WPRIM
Type of study:
Diagnostic_studies
Language:
Zh
Journal:
Chinese Journal of Clinical Laboratory Science
Year:
2006
Type:
Article