Polylatic acid microspheres-induced CTL cytotoxicity against hepatocellular carcinoma cell lines / 中国肿瘤生物治疗杂志
Chinese Journal of Cancer Biotherapy
;
(6)1995.
Article
in Chinese
| WPRIM
| ID: wpr-586837
ABSTRACT
Objective:
To investigate the in vitro efficiency of two kinds of polylatic acid (PLA) immunomicrospheres M1(hAFP158~166)and M2(hAFP218~226) in specific inducement of CTL and the cytotoxicity of CTL against hepatocellular carcinoma cells.Methods:
Pripheral blood monocytes (PBMC) of HLA-A2+ healthy volunteers stimulated in vitro by peptide-loading microspheres were taken as effectors. The experiments were divided into three groups control group, hAFP+ tumor cells group, and hAFP- tumor cells group. The specific cytolysis of target cells was assessed by 51Cr-release assay.Results:
Both M1 and M2 induced proliferation of HLA-A2+ PBMC, forming visible clones. The effector cells induced by M1 and M2 both had an cytolysis rate over 75% for hAFP+ tumor cells, which was significantly higher than that for hAFP- tumor cells (P0.05).Conclusion:
The two kinds of microspheres can both induce specific CTL in vitro, which can effectively kill hAFP+ tumor cells.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Cancer Biotherapy
Year:
1995
Type:
Article
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