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Effects of Recombinant Angiotensin-converting enzyme 2 Gene on Oxidative Stress Levels in Human Endothelial Cells / 中华高血压杂志
Article in Zh | WPRIM | ID: wpr-591777
Responsible library: WPRO
ABSTRACT
Backgroup and objective Angiotensin-converting enzyme 2 (ACE2) is the first known homologue of human ACE gene up till now,however,its regulatory role in oxidative stress remains unclear.Our aim is to in- vestigate the effects of recombinant ACE2 gene transfer on the expression of p22~(phox),a key subunit of NADPH oxi- dase,and malonyldialdehyde (MDA) levels induced by angiotension(Ang) Ⅱ in cultured human endothelial cells. Methods A recombinant plasmid encompassing human ACE2 gene (pACE2) was constructed and transfected into these cells.The mRNA and protein levels of p22~(phox) in endothelial cells were determined by real-time PCR and Western blotting,respectively.MDA contents were measured by thiobarbiturie acid colorimetrie method in cells. Results The mRNA and protein expressions of p22~(phox) were drastically enhanced after exposures of endothelial cells to Ang Ⅱ(100 nmol/L) and Ang Ⅳ(100 nmol/L),accompanied by an increase in MDA contents (n=6,P
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Full text: 1 Index: WPRIM Language: Zh Journal: Chinese Journal of Hypertension Year: 2007 Type: Article
Full text: 1 Index: WPRIM Language: Zh Journal: Chinese Journal of Hypertension Year: 2007 Type: Article