IL-10 reduces Interleukin-1? in rats with acute necrotizing pancreatitis / 基础医学与临床

ABSTRACT

Objective To investigate the effects of recombinant human interleukin(IL)-10 on serum interleukin-1? in L-arginine-induced acute necrotizing pancreatitis of rats.Methods Ninty-two Sprague-Dawley rats were randomly divided into three groups.Group A(n=36) and Group I(n=32) received three intraperitoneal injections of 6% L-arginine(1.0 mg/g) at hourly intervals.Group I(n=32) was treated with 10,000 unites of intraperitioneal recombinant human IL-10 at the 2nd,5th and 8th hour after the last injection of L-arginine.Group C(n=24) received saline alone.Rats were killed at the 4th,12th,24th and 36th hour after the last L-arginine injection.Serum interleukin-1? and amylase were assayed.Pancreatic tissues were examined histopathologically by microscopy.Results Serum amylase,interleukin-1? and pancreatic histopathological scores in group A increased significantly after L-arginine injection(P