MAR increased transgene expression in stable transfected CHO cells / 基础医学与临床
Basic & Clinical Medicine
; (12)2006.
Article
in Zh
| WPRIM
| ID: wpr-593095
Responsible library:
WPRO
ABSTRACT
Objective To study the effect of human ?-globin matrix attachment region(MAR) on transgene expression in stably transfected CHO cells.Methods Expression vector was constructed,which contained the ?-globin MAR in both sides of Chloramphenicol acetyltransferase(CAT) reporter gene expression cassette in cis,then transfected into CHO cells.The CAT reporter gene expression was analyzed by ELISA method.Results The ?-globin MAR enhanced the CAT gene expression 5.5-fold in stably transformed CHO cells,while the transgene expression variation among individuals of transformants was decreased.Conclusion MAR increase transgene expression in stably transfected CHO cells.
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WPRIM
Language:
Zh
Journal:
Basic & Clinical Medicine
Year:
2006
Type:
Article