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HER-2 RNA interference improves sensitivity of breast cancer cells to chemotherapy in vitro and in vivo / 中国肿瘤生物治疗杂志
Chinese Journal of Cancer Biotherapy ; (6)1995.
Article in Chinese | WPRIM | ID: wpr-594609
ABSTRACT

Objective:

To observe the sensitivities of breast cancer cells and its implanted tumors to chemotherapeutic drug epirubicin after down-regulation of HER-2 expression by RNA interference(RNAi).

Methods:

HER-2siRNApU6 vector containing HER-2 RNAi was constructed and was used to transfect HER-2 positive breast cancer cell line SKBR-3.The expression of HER-2 mRNA and protein were analyzed by RT-PCR and Western blotting,respectively.Transfected SKBR-3 cells were treated with different concentrations of epirubicin;the growth of SKBR-3 cells and IC50 of epirubicin were observed by MTT.SKBR-3 cells were injected into nude mouse to establish breast cancer model;the sensitivity of mouse model to epirubicin was observed after HER-2shRNApU6 treatment.

Results:

Expression of HER-2 mRNA and HER-2 protein were down-regulated in SKBR-3 cells after transfection with HER-2shRNApU6.Furthermore,the proliferation of SKBR-3 cells transfected with HER-2shRNApU6 was significantly decreased compared with mock tansfected group(P

Full text: Available Index: WPRIM (Western Pacific) Type of study: Diagnostic study Language: Chinese Journal: Chinese Journal of Cancer Biotherapy Year: 1995 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Type of study: Diagnostic study Language: Chinese Journal: Chinese Journal of Cancer Biotherapy Year: 1995 Type: Article