Adipose Gene Expression Profiles Related to Metabolic Syndrome Using Microarray Analyses in Two Different Models
Diabetes & Metabolism Journal
; : 356-365, 2014.
Article
in En
| WPRIM
| ID: wpr-59599
Responsible library:
WPRO
ABSTRACT
BACKGROUND:
Peroxisome proliferator-activated receptor-gamma (PPAR-gamma) agonist has a wide-ranging influence on multiple components of metabolic syndrome. The Otsuka Long-Evans Tokushima Fatty (OLETF) rat is a useful animal model of metabolic syndrome. To determine genes related to metabolic syndrome, we examined overlapping genes that are simultaneously decreased by PPAR-gamma agonists and increased in OLETF rats using microarrays in two different models.METHODS:
In the first microarray analysis, PPAR-gamma agonist-treated db/db mice were compared to standard diet-fed db/db mice. In the second microarray analysis, OLETF rats were compared to Long-Evans Tokushima Otsuka (LETO) rats (control of OLETF rats).RESULTS:
Among the overlapping genes, in the present study, we validated that lipocalin-2 expression was significantly decreased in the visceral adipose tissue of PPAR-gamma agonist-treated db/db mice compared to standard diet-fed db/db mice and increased in OLETF rats compared to LETO rats using real time reverse transcription polymerase chain reaction. Furthermore, we showed for the first time that lipocalin-2 expression was significantly increased in the visceral adipose tissues of obese humans compared with nonobese humans. In addition, the expression level of lipocalin-2 in human visceral adipose tissue had a significant positive correlation with body mass index, serum interleukin-6, adipocyte fatty acid binding protein levels, and white blood cell count.CONCLUSION:
Lipocalin-2 was confirmed to be a significant adipokine affected by PPAR-gamma agonist and obesity in the present study. Also, for the first time in human visceral adipose tissue, it was determined that the expression of lipocalin-2 from obese humans was significantly increased and correlated with circulating inflammatory markers.Key words
Full text:
1
Index:
WPRIM
Main subject:
Genes, Overlapping
/
Carrier Proteins
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Body Mass Index
/
Polymerase Chain Reaction
/
Interleukin-6
/
Adipocytes
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Rats, Inbred OLETF
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Peroxisomes
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Models, Animal
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PPAR gamma
Type of study:
Prognostic_studies
Limits:
Animals
/
Humans
Language:
En
Journal:
Diabetes & Metabolism Journal
Year:
2014
Type:
Article